Premium
Mitochondrial biogenesis and increased uncoupling protein 1 in brown adipose tissue of mice fed a ketone ester diet
Author(s) -
Srivastava Shireesh,
Kashiwaya Yoshihiro,
King M. Todd,
Baxa Ulrich,
Tam Joseph,
Niu Gang,
Chen Xiaoyuan,
Clarke Kieran,
Veech Richard L.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.11-200410
Subject(s) - thermogenin , medicine , brown adipose tissue , endocrinology , uncoupling protein , mitochondrial biogenesis , adipose tissue , ketone bodies , biology , leptin , mitochondrion , chemistry , metabolism , biochemistry , obesity
We measured the effects of a diet in which D‐β‐hydroxybutyrate‐( R )‐1,3 butanediol monoester [ketone ester (KE)] replaced equicaloric amounts of carbohydrate on 8‐wk‐old male C57BL/6J mice. Diets contained equal amounts of fat, protein, and micronutrients. The KE group was fed ad libitum , whereas the control (Ctrl) mice were pair‐fed to the KE group. Blood D‐β‐hydroxybutyrate levels in the KE group were 3‐5 times those reported with high‐fat ketogenic diets. Voluntary food intake was reduced dose dependently with the KE diet. Feeding the KE diet for up to 1 mo increased the number of mitochondria and doubled the electron transport chain proteins, uncoupling protein 1, and mitochondrial biogenesis‐regulating proteins in the interscapular brown adipose tissue (IBAT). [ 18 F]‐Fluorodeoxyglucose uptake in IBAT of the KE group was twice that in IBAT of the Ctrl group. Plasma leptin levels of the KE group were more than 2‐fold those of the Ctrl group and were associated with increased sympathetic nervous system activity to IBAT. The KE group exhibited 14% greater resting energy expenditure, but the total energy expenditure measured over a 24‐h period or body weights was not different. The quantitative insulin‐sensitivity check index was 73% higher in the KE group. These results identify KE as a potential antiobesity supplement.—Srivastava, S., Kashiwaya, Y., King, M. T. Baxa, U., Tam, J., Niu, G., Chen, X., Clarke, K., Veech, R. L. Mitochondrial biogenesis and increased uncoupling protein 1 in brown adipose tissue of mice fed a ketone ester diet. FASEB J. 26, 2351‐2362 (2012). www.fasebj.org