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Binding of galectin‐1 to α IIb β 3 integrin triggers “outside‐in” signals, stimulates platelet activation, and controls primary hemostasis
Author(s) -
Romaniuk Maria A.,
Croci Diego O.,
Lapponi Maria J.,
Tribulatti Maria V.,
Negrotto Soledad,
Poirier Francoise,
Campetella Oscar,
Rabinovich Gabriel A.,
Schattner Mirta
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.11-197541
Subject(s) - platelet , integrin , chemistry , platelet activation , platelet glycoprotein gpiib iiia complex , clot retraction , gpvi , collagen receptor , hemostasis , syk , thromboxane a2 , microbiology and biotechnology , receptor , biochemistry , thrombin , immunology , medicine , biology , tyrosine kinase
Understanding noncanonical mechanisms of platelet activation represents an important challenge for the identification of novel therapeutic targets in bleeding disorders, thrombosis, and cancer. We previously reported that galectin‐1 (Gal‐1), a β‐galactoside‐binding protein, triggers platelet activation in vitro. Here we investigated the molecular mechanisms underlying this function and the physiological relevance of endogenous Gal‐1 in hemostasis. Mass spectrometry analysis, as well as studies using blocking antibodies against the anti‐α IIb subunit of α IIb β 3 integrin or platelets from patients with Glanzmann's thrombasthenia syndrome (α IIb β 3 deficiency), identified this integrin as a functional Gal‐1 receptor in platelets. Binding of Gal‐1 to platelets triggered the phosphorylation of β 3 ‐integrin, Syk, MAPKs, PI3K, PLCγ2, thromboxane (TXA 2 ) release, and Ca 2+ mobilization. Not only soluble but also immobilized Gal‐1 promoted platelet activation. Gal‐1‐deficient ( Lgals1 –/– ) mice showed increased bleeding time ( P < 0.0002, knockout vs. wild type), which was not associated with an abnormal platelet count. Lgals1 –/– platelets exhibited normal aggregation to PAR4, ADP, arachidonic acid, or collagen but abnormal ATP release at low collagen concentrations. Impaired spreading on fibrinogen and clot retraction with normal levels of α IIb β3 was also observed in Lgals1 –/– platelets, indicating a failure in the “outside‐in” signaling through this integrin. This study identifies a noncanonical mechanism, based on galectin‐integrin interactions, for regulating platelet activation.—Romaniuk, M. A., Croci, D. O., Lapponi, M. J., Tribulatti, M. V., Negrotto, S., Poirier, F., Campetella, O., Rabinovich, G. A., Schattner, M. Binding of galectin‐1 to α IIb β 3 integrin triggers “outside‐in” signals, stimulates platelet activation, and controls primary hemostasis. FASEB J. 26, 2788–2798 (2012). www.fasebj.org