Premium
DNA methylation profiling at imprinted loci after periconceptional micronutrient supplementation in humans: results of a pilot randomized controlled trial
Author(s) -
Cooper Wendy N.,
Khulan Batbayar,
Owens Stephen,
Elks Cathy E.,
Seidel Veronica,
Prentice Andrew M.,
Belteki Gusztav,
Ong Ken K.,
Affara Nabeel A.,
Constância Miguel,
Dunger David B.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.11-192708
Subject(s) - offspring , micronutrient , dna methylation , epigenome , differentially methylated regions , randomized controlled trial , biology , physiology , pregnancy , context (archaeology) , endocrinology , medicine , genetics , gene , pathology , paleontology , gene expression
Intrauterine exposures mediated by maternal diet may affect risk of cardiovascular disease, obesity, and type 2 diabetes. Recent evidence, primarily from animal studies and observational data in humans, suggests that the epigenome can be altered by maternal diet during the periconceptional period and that these programming events may underlie later disease risk. A randomized controlled trial of periconceptional micronutrient supplementation in The Gambia, where seasonal nutritional variations affect fetal growth and postnatal outcomes, provided a unique opportunity to test this hypothesis. Specifically, we targeted imprinted genes, which play important roles in allocation of maternal resources while being epigenetically regulated. DNA methylation at 12 differentially methylated regions (DMRs) was analyzed in cord blood samples from 58 offspring of women participating in a doubleblind randomized‐controlled trial of pre‐ and periconceptional micronutrient supplementation (including folate, zinc, and vitamins A, B, C, and D). We observed sex‐specific effects of micronutrient supplementation, reducing methylation levels at two of the DMRs analyzed, IGF2R in girls and GTL2 ‐2 in boys. This pilot study is the first to analyze DNA methylation in the context of a randomized controlled trial, and it provides suggestive evidence that periconceptional maternal nutrition alters offspring methylation at imprinted loci.—Cooper, W. N., Khulan, B., Owens, S., Elks, C. E., Seidel, V., Prentice, A. M., Belteki, G., Ong, K. K., Affara, N. A., Constância, M., Dunger, D. B. DNA methylation profiling at imprinted loci after periconceptional micronutrient supplementation in humans: results of a pilot randomized controlled trial. FASEB J. 26, 1782‐1790 (2012). www.fasebj.org