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Dietary fructose inhibits lactation‐induced adaptations in rat 1,25‐(OH) 2 D 3 synthesis and calcium transport
Author(s) -
Douard Veronique,
Suzuki Takuji,
Sabbagh Yves,
Lee Jacklyn,
Shapses Sue,
Lin Sheldon,
Ferraris Ronaldo P.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.11-190264
Subject(s) - lactation , fructose , medicine , endocrinology , chemistry , parathyroid hormone , calcium , homeostasis , biochemistry , biology , pregnancy , genetics
We recently showed that excessive fructose consumption, already associated with numerous metabolic abnormalities, reduces rates of intestinal Ca 2+ transport. Using a rat lactation model with increased Ca 2+ requirements, we tested the hypothesis that mechanisms underlying these inhibitory effects of fructose involve reductions in renal synthesis of 1,25‐(OH) 2 D 3 . Pregnant and virgin (control) rats were fed isocaloric fructose or, as controls, glucose, and starch diets from d 2 of gestation to the end of lactation. Compared to virgins, lactating dams fed glucose or starch had higher rates of intestinal trans‐cellular Ca 2+ transport, elevated intestinal and renal expression of Ca 2+ channels, Ca 2+ ‐binding proteins, and CaATPases, as well as increased levels of 25‐(OH)D 3 and 1,25‐(OH) 2 D 3 . Fructose consumption prevented almost all of these lactation‐induced increases, and reduced vitamin D receptor binding to promoter regions of Ca 2+ channels and binding proteins. Changes in 1,25‐(OH) 2 D 3 level were tightly correlated with alterations in expression of 1α‐hydroxylase but not with levels of parathyroid hormone and of 24‐hydroxylase. Bone mineral density, content, and mechanical strength each decreased with lactation, but then fructose exacerbated these effects. When Ca 2+ requirements increase during lactation or similar physiologically challenging conditions, excessive fructose consumption may perturb Ca 2+ homeostasis because of fructose‐induced reductions in synthesis of 1,25‐(OH) 2 D 3 .—Douard, V., Suzuki, T., Sabbagh, Y., Lee, J., Shapses, S., Lin, S., Ferraris, R. P. Dietary fructose inhibits lactation‐induced adaptations in rat 1,25‐(OH) 2 D 3 synthesis and calcium transport. FASEB J. 26, 707–721 (2012). www.fasebj.org