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Functional characterization connects individual patient mutations in ataxia telangiectasia mutated (ATM) with dysfunction of specific DNA double‐strand break‐repair signaling pathways
Author(s) -
Keimling Marlen,
Volcic Meta,
Csernok Andreea,
Wieland Britta,
Dörk Thilo,
Wiesmüller Lisa
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.11-185546
Subject(s) - ataxia telangiectasia , non homologous end joining , rad50 , dna repair , dna damage , biology , genetics , mutation , homologous recombination , ku80 , microbiology and biotechnology , dna , cancer research , gene , dna binding protein , transcription factor
Ataxia telangiectasia mutated (ATM) has multiple functions in homologous recombination (HR) and nonhomologous end joining (NHEJ), which lead to conflicting data regarding its DNA double‐strand break‐repair (DSBR) functions in previous studies. To explore the effect of clinically relevant ATM mutations, we characterized DSBR between mutated EGFP genes and ATM kinase signaling in 9 lymphoblastoid cell lines (LCLs) derived from patients with ataxia telangiectasia (AT) with defined vs. 3 control LCLs without ATM mutations. Our study revealed that the DSBR phenotype in AT cells is not uniform but appears to depend on the mutation, causing up to 32‐fold increased or up to 3‐fold decreased activities in particular pathways. Comparison with a further 10 LCLs mutated in downstream factors (BRCA1, BRCA2, Nibrin, Rad50, and Chk2) showed that the most diametrically opposed DSBR patterns in AT cells phenocopied NBN/RAD50 or BRCA1 mutations. Notably, reexpressing wild‐type ATM reversed these defects by 2.3‐ to 3.5‐fold. Our data suggest that ATM stimulates repair proteins such as Nibrin, which execute HR, single‐strand annealing (SSA), and NHEJ. Concomitantly, ATM minimizes error‐prone repair (SSA and NHEJ) through activation of surveillance factors such as BRCA1. Since the outcome of the individual defect can be diametrically opposed, distinguishing repair patterns in patients with ATM mutations may also be relevant regarding therapeutic responses.—Keimling, M., Volcic, M., Csernok, A., Wieland, B., Dörk, T., Wiesmüller, L. Functional characterization connects individual patient mutations in ataxia telangiectasia mutated (ATM) with dysfunction of specific DNA double‐strand break‐repair signaling pathways. FASEB J. 25, 3849–3860 (2011). www.fasebj.org