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Dietary selenium affects host selenoproteome expression by influencing the gut microbiota
Author(s) -
Kasaikina Marina V.,
Kravtsova Marina A.,
Lee Byung Cheon,
Seravalli Javier,
Peterson Daniel A.,
Walter Jens,
Legge Ryan,
Benson Andrew K.,
Hatfield Dolph L.,
Gladyshev Vadim N.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.11-181990
Subject(s) - selenium , biology , selenium deficiency , gut flora , glutathione peroxidase , gastrointestinal tract , host (biology) , selenocysteine , population , microbiology and biotechnology , biochemistry , chemistry , glutathione , enzyme , ecology , demography , organic chemistry , cysteine , sociology
Colonization of the gastrointestinal tract and composition of the microbiota may be influenced by components of the diet, including trace elements. To understand how selenium regulates the intestinal microflora, we used high‐throughput sequencing to examine the composition of gut microbiota of mice maintained on selenium‐deficient, selenium‐sufficient, and selenium‐enriched diets. The microbiota diversity increased as a result of selenium in the diet. Specific phylotypes showed differential effects of selenium, even within a genus, implying that selenium had unique effects across microbial taxa. Conventionalized germ‐free mice subjected to selenium diets gave similar results and showed an increased diversity of the bacterial population in animals fed with higher levels of selenium. Germ‐free mice fed selenium diets modified their selenoproteome expression similar to control mice but showed higher levels and activity of glutathione peroxidase 1 and methionine‐R‐sulfoxide reductase 1 in the liver, suggesting partial sequestration of selenium by the gut microorganisms, limiting its availability for the host. These changes in the selenium status were independent of the levels of other trace elements. The data show that dietary selenium affects both composition of the intestinal microflora and colonization of the gastrointestinal tract, which, in turn, influence the host selenium status and selenoproteome expression.—Kasaikina, M. V., Kravtsova, M. A., Lee, B. C., Seravalli, J., Peterson, D. A., Walter, J., Legge, R., Benson, A. K., Hatfield, D. L., Gladyshev, V. N. Dietary selenium affects host selenoproteome expression by influencing the gut microbiota. FASEB J. 25, 2492–2499 (2011). www.fasebj.org