z-logo
Premium
Chromogranins A and B are key proteins in amine accumulation, but the catecholamine secretory pathway is conserved without them
Author(s) -
DíazVera Jésica,
Camacho Marcial,
Machado José David,
Domínguez Natalia,
Montesinos Mónica S.,
HernándezFernaud Juan R.,
Luján Rafael,
Borges Ricardo
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.11-181941
Subject(s) - secretory vesicle , vesicle , exocytosis , secretory protein , microbiology and biotechnology , biogenesis , secretory pathway , biology , adrenal medulla , secretion , biochemistry , chemistry , catecholamine , endoplasmic reticulum , endocrinology , gene , membrane , golgi apparatus
Chromogranins are the main soluble proteins in the large dense core secretory vesicles (LDCVs) found in aminergic neurons and chromaffin cells. We recently demonstrated that chromogranins A and B each regulate the concentration of adrenaline in chromaffin granules and its exocytosis. Here we have further studied the role played by these proteins by generating mice lacking both chromogranins. Surprisingly, these animals are both viable and fertile. Although chromogranins are thought to be essential for their biogenesis, LDCVs were evident in these mice. These vesicles do have a somewhat atypical appearance and larger size. Despite their increased size, single‐cell amperometry recordings from chromaffin cells showed that the amine content in these vesicles is reduced by half. These data demonstrate that although chromogranins regulate the amine concentration in LDCVs, they are not completely essential, and other proteins unrelated to neurosecretion, such as fibrinogen, might compensate for their loss to ensure that vesicles are generated and the secretory pathway conserved.—Díaz‐Vera, J., Camacho, M., Machado, J. D., Domínguez, N., Montesinos, M. S., Hernández‐Fernaud, J. R., Luján, R., Borges, R. Chromogranins A and B are key proteins in amine accumulation, but the catecholamine secretory pathway is conserved without them. FASEB J. 26, 430–438 (2012). www.fasebj.org

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here