Premium
Sequenced response of extracellular matrix deadhesion and fibrotic regulators after muscle damage is involved in protection against future injury in human skeletal muscle
Author(s) -
Mackey Abigail L.,
Brandstetter Simon,
Schjerling Peter,
BojsenMoller Jens,
Qvortrup Klaus,
Pedersen Mette M.,
Doessing Simon,
Kjaer Michael,
Magnusson S. Peter,
Langberg Henning
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.10-176487
Subject(s) - skeletal muscle , extracellular matrix , tenascin c , gastrocnemius muscle , laminin , tenascin , stimulation , biology , inflammation , cardiotoxin , heat shock protein , microbiology and biotechnology , medicine , endocrinology , pathology , immunology , biochemistry , gene , fibronectin
The purpose of this study was to test the hypothesis that remodeling of skeletal muscle extracellular matrix (ECM) is involved in protecting human muscle against injury. Biopsies were obtained from medial gastrocnemius muscles after a single bout of electrical stimulation (B) or a repeated bout (RB) 30 d later, or 30 d after a single stimulation bout (RBc). A muscle biopsy was collected from the control leg for comparison with the stimulated leg. Satellite cell content, tenascin C, and muscle regeneration were assessed by immunohistochemistry; real‐time PCR was used to measure mRNA levels of collagens, laminins, heat‐shock proteins (HSPs), inflammation, and related growth factors. The large responses of HSPs, CCL2, and tenascin C detected 48 h after a single bout were attenuated in the RB trial, indicative of protection against injury. Satellite cell content and 12 target genes, including IGF‐1, were elevated 30 d after a single bout. Among those displaying the greatest difference vs. control muscle, ECM laminin‐β1 and collagen types I and III were elevated ~6‐ to 9‐fold ( P< 0.001). The findings indicate that the sequenced events of load‐induced early deadhesion and later strengthening of skeletal muscle ECM play a role in protecting human muscle against future injury.—Mackey, A. L., Brandstetter, S., Schjerling, P., Bojsen‐Moller, J., Qvortrup, K., Pedersen, M. M., Doessing, S. Kjaer, M., Magnus‐son, S. P., Langberg, H. Sequenced response of extracellular matrix deadhesion and fibrotic regulators after muscle damage is involved in protection against future injury in human skeletal muscle. FASEB J. 25, 1943‐1959 (2011). www.fasebj.org