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Lipid membrane editing with peptide cargo linkers in cells and synthetic nanostructures
Author(s) -
Pan Hua,
Myerson Jacob W.,
Ivashyna Olena,
Soman Neelesh R.,
Marsh Jon N.,
Hood Joshua L.,
Lanza Gregory M.,
Schlesinger Paul H.,
Wickline Samuel A.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.09-153130
Subject(s) - nanocarriers , peptide , linker , in vivo , chemistry , drug delivery , nanotechnology , targeted drug delivery , combinatorial chemistry , biophysics , computational biology , biochemistry , computer science , materials science , biology , microbiology and biotechnology , operating system
ABSTRACT Current strategies for deploying synthetic nanocarriers involve the creation of agents that incorporate targeting ligands, imaging agents, and/or therapeutic drugs into particles as an integral part of the formulation process. Here we report the development of an amphipathic peptide linker that enables postformulation editing of payloads without the need for reformulation to achieve multiplexing capability for lipidic nanocarriers. To exemplify the flexibility of this peptide linker strategy, 3 applications were demonstrated: converting nontargeted nanoparticles into targeting vehicles;adding cargo to preformulated targeted nanoparticles for in vivo site‐specific delivery;and labeling living cells for in vivo tracking. This strategy is expected to enhance the clinical application of molecular imaging and/or targeted therapeutic agents by offering extended flexibility for multiplexing targeting ligands and/or drug payloads that can be selected after base nanocarrier formulation.—Pan, H., Myerson, J. W., Ivashyna, O., Soman, N. R., Marsh, J. N., Hood, J. L., Lanza, G. M., Schlesinger, P. H., Wickline, S. A.. Lipid membrane editing with peptide cargo linkers in cells and synthetic nanostructures. FASEB J . 24, 2928–2937 (2010). www.fasebj.org