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Myocardial knockdown of mRNA‐stabilizing protein HuR attenuates post‐MI inflammatory response and left ventricular dysfunction in IL‐10‐null mice
Author(s) -
Krishnamurthy Prasanna,
Lambers Erin,
Verma Suresh,
Thorne Tina,
Qin Gangjian,
Losordo Douglas W.,
Kishore Raj
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.09-149815
Subject(s) - gene knockdown , messenger rna , inflammatory response , medicine
Prolonged inflammatory response is as sociated with left ventricular (LV) dysfunction and adverse remodeling following myocardial infarction (MI). IL‐10 inhibits inflammation by suppressing HuR‐mediated mRNA stabilization of proinflammatory cytokines. Here we report that following MI, IL‐10 −/− mice showed exaggerated LV dysfunction, fibrosis, and cardiomyocyte apoptosis. Short‐hairpin RNA ( shRNA )‐mediated knockdown of HuR in the myocardium significantly reversed MI‐induced LV dysfunctions and LV remodeling. HuR knockdown significantly reduced MI‐induced cardiomyocyte apoptosis concomitant with reduced p53 expression. Moreover, HuR knockdown significantly reduced infarct size and fibrosis area, which in turn was associated with decreased TGF‐ß expression. In vitro , stable knockdown of HuR in mouse macrophage cell line RAW 264.7 corroborated in vivo data and revealed reduced mRNA expression of TNF‐α, TGF‐ß, and p53 following LPS challenge, which was associated with a marked reduction in the mRNA stability of these genes. Taken together, our studies suggest that HuR is a direct target of IL‐10, and HuR knockdown mimics anti‐inflammatory effects of IL10.—Krishnamurthy, P., Lambers, E., Verma, S., Thorne, T., Qin, G., Losordo, D. W., Kishore, R. Myocardial knockdown of mRNA‐stabilizing protein HuR attenuates post‐MI inflammatory response and left ventricular dysfunction in IL‐10‐null mice. FASEB J. 24, 2484–2494 (2010). www.fasebj.org