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A versatile system for the neuronal subtype specific expression of lentiviral vectors
Author(s) -
Tolu Stefania,
Avale M. Elena,
Nakatani Hiroko,
Pons Stephanie,
Parnaudeau Sebastien,
Tronche Francois,
Vogt Angelika,
Monyer Hannah,
Vogel Roland,
Chaumont Fabrice,
OlivoMarín JeanChristophe,
Changeux JeanPierre,
Maskos Uwe
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.09-139790
Subject(s) - transgene , viral vector , transduction (biophysics) , cre recombinase , biology , microbiology and biotechnology , reporter gene , gene delivery , genetic enhancement , gene expression , gene , genetically modified mouse , genetics , recombinant dna , biochemistry
Lentiviral expression vectors are powerful tools for gene therapy and long‐term gene expression/repression in the mammalian brain. However, no specificity of transduction has been reported so far in the central nervous system. Here we have developed a novel system to achieve a neuronal subtype specific expression in either dopaminergic (DA) or GABAergic neurons. We employed a delivery strategy by which the transgene is not expressed until its activation by Cre recombinase. We successfully tested the system in vitro and then used this novel lentivector, containing lox P sites, in 2 different transgenic mouse lines expressing Cre either in DA or in GABAergic neurons. In both lines the reporter gene was detected exclusively in Cre‐positive cells, demonstrating that with this experimental approach we were able to achieve completely specific expression of transgenes delivered by lentiviral vectors. This universal system can be applied to all neural subtypes making use of the growing number of specific Cre driver lines.—Tolu, S., Avale, M. E., Nakatani, H., Pons, S., Parnaudeau, S., Tronche, F., Vogt, A., Monyer, H., Vogel, R., de Chaumont, F., Olivo‐Marin, J.‐C., Changeux, J.‐P., Maskos, U. A versatile system for the neuronal subtype specific expression of lentiviral vectors. FASEB J. 24, 723–730 (2010). www.fasebj.org