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Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells
Author(s) -
Mizuno Yuta,
Chang Hsi,
Umeda Katsutsugu,
Niwa Akira,
Iwasa Toru,
Awaya Tomonari,
Fukada Soichiro,
Yamamoto Hiroshi,
Yamanaka Shinya,
Nakahata Tatsutoshi,
Heike Toshio
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.09-137174
Subject(s) - induced pluripotent stem cell , stem cell , embryonic stem cell , microbiology and biotechnology , progenitor cell , adult stem cell , induced stem cells , transplantation , cell sorting , biology , skeletal muscle , immunology , anatomy , medicine , flow cytometry , genetics , gene
Induced pluripotent stem (iPS) cells, which are a type of pluripotent stem cell generated from reprogrammed somatic cells, are expected to have potential for patient‐oriented disease investigation, drug screening, toxicity tests, and transplantation therapies. Here, we demonstrated that murine iPS cells have the potential to develop in vitro into skeletal muscle stem/progenitor cells, which are almost equivalent to murine embryonic stem cells. Cells with strong in vitro myogenic potential effectively were enriched by fluorescence‐activated cell sorting using the anti‐satellite cell antibody SM/C‐2.6. Furthermore, on transplantation into mdx mice, SM/C‐2.6 + cells exerted sustained myogenic lineage differentiation in injured muscles, while providing long‐lived muscle stem cell support. Our data suggest that iPS cells have the potential to be used in clinical treatment of muscular dystrophies.—Mizuno, Y., Chang, H., Umeda, K., Niwa, A, Iwasa, T., Awaya, T., Fukada, S., Yamamoto, H., Yamanaka, S., Nakahata, T., Heike, T. Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells. FASEB J. 24, 2245–2253 (2010). www.fasebj.org