Premium
Pluripotent stem cells are highly susceptible targets for syngeneic, allogeneic, and xenogeneic natural killer cells
Author(s) -
Dressel Ralf,
Nolte Jessica,
Elsner Leslie,
Novota Peter,
Guan Kaomei,
StreckfussBömeke Katrin,
Hasenfuss Gerd,
Jaenisch Rudolf,
Engel Wolfgang
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.09-134957
Subject(s) - induced pluripotent stem cell , stem cell , immunology , biology , microbiology and biotechnology , embryonic stem cell , gene , genetics
Multipotent adult germ‐line stem cells (maGSCs) and induced pluripotent stem cells (iPSCs) could be used to generate autologous cells for therapeutic purposes, which are expected to be tolerated by the recipient. However, effects of the immune system on these cells have not been investigated. We have compared the susceptibility of maGSC lines to IL‐2‐activated natural killer (NK) cells with embryonic stem cell (ESC) lines, iPSCs, and F9 teratocarcinoma cells. The killing of pluripotent cell lines by syngeneic, allogeneic, and xenogeneic killer cells ranged between 48 and 265% in chromium release assays when compared to YAC‐1 cells, which served as highly susceptible reference cells. With the exception of 2 maGSC lines, they expressed ligands for the activating NK receptor NKG2D that belong to the RAE‐1 family, and killing could be inhibited by soluble NKG2D, demonstrating a functional role of these molecules. Furthermore, ligands of the activating receptor DNAM‐1 were frequently expressed. The susceptibility to NK cells might constitute a common feature of pluripotent cells. It could result in rejection after transplantation, as suggested by a reduced teratoma growth after NK cell activation in vivo , but it might also offer a strategy to deplete contaminating pluripotent cells before grafting of differentiated cells.—Dressel, R., Nolte, J., Elsner, L., Novota, P., Guan, K, Streckfuss‐Bömeke, K, Hasenfuss, G., Jaenisch, R., Engel, W. Pluripotent stem cells are highly susceptible targets for syngeneic, allogeneic, and xenogeneic natural killer cells. FASEBJ. 24, 2164–2177 (2010). www.fasebj.org