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Adenosine 2B receptors (A 2B AR) on enteric neurons regulate murine distal colonic motility
Author(s) -
Chandrasekharan Bindu P.,
Kolachala Vasantha L.,
Dalmasso Guillaume,
Merlin Didier,
Ravid Katya,
Sitaraman Shanthi V.,
Srinivasan Shanthi
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.09-129544
Subject(s) - motility , distal colon , receptor , chemistry , gastric emptying , enteric nervous system , stimulation , myenteric plexus , medicine , adenosine , endocrinology , nitric oxide , pharmacology , biology , microbiology and biotechnology , immunohistochemistry , stomach
Delayed colonic emptying leading to constipation is a significant health concern. We investigated the role of adenosine 2B receptor (A 2B AR) in modulating distal colonic motility using wild‐type and A 2B AR‐knockout ( A 2B AR –/– ) mice. Colon motility was assessed using stool characteristics and colonic transit. Distal colonic ganglia, isolated by laser capture microdissection, were tested for A 2B AR expression by RT‐ PCR. The distal colon contraction and relaxation responses were assessed by electrical field stimulation (EFS) in presence of A 2B AR agonists, antagonists or inhibitors of nitric oxide (NO) and guanylate cyclase. Nitrite levels were measured in enteric neuronal cultures exposed to A 2B AR agonists/antagonists. A 2B AR –/– mice display increased stool retention, decreased stool frequency, delayed colonic emptying, and decreased circular muscle relaxation. RT‐PCR identified A 2B AR expression in distal colonic ganglia. EFS studies revealed that enteric neuronal A 2B AR is essential for distal colonic relaxation, and A 2B AR antagonists can inhibit relaxation. Enteric neurons stimulated with A 2B AR agonists produced more nitrite than cultures treated with antagonists. We demonstrate an essential role of A 2B AR in regulating distal colon relaxation, as A 2B AR activation is linked to NO signaling. Hence targeting the colonic A 2B AR could represent a novel therapeutic strategy to treat constipation.— Chandrasekharan, B. P., Kolachala, V. L., Dalmasso, G., Merlin, D., Ravid, K., Sitaraman, S. V., Srinivasan, S. Adenosine 2B receptors (A2BAR) on enteric neurons regulate murine distal colonic motility. FASEB J. 23, 2727–2734 (2009)