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Embryonic caffeine exposure induces adverse effects in adulthood
Author(s) -
Wendler Christopher C.,
Busovsky-McNeal Melissa,
Ghatpande Satish,
Kalinowski April,
Russell Kerry S.,
Rivkees Scott A.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.08-124941
Subject(s) - caffeine , offspring , hypoxia (environmental) , in utero , saline , endocrinology , medicine , cardiac function curve , embryo , fetus , chemistry , pregnancy , biology , oxygen , heart failure , organic chemistry , microbiology and biotechnology , genetics
The purpose of this study was to determine both the short‐term effects on cardiac development and embryo growth and the long‐term effects on cardiac function and body composition of in utero caffeine exposure. Pregnant mice (C57BL/6) were exposed to hypoxia (10% O 2 ) or room air from embryonic days (E) 8.5–10.5, and treated with caffeine (20 mg/kg, i.p.) or vehicle (normal saline, 0.9% NaCl). This caffeine dose results in a circulating level that is equivalent to 2 cups of coffee in humans. Hypoxic exposure acutely reduced embryonic growth by 30%. Exposure to a single dose of caffeine inhibited cardiac ventricular development by 53% in hypoxia and 37% in room air. Caffeine exposure resulted in inhibition of hypoxia‐induced HIF1α protein expression in embryos by 40%. When offspring from dams treated with a single dose of caffeine were studied in adulthood, we observed that caffeine treatment alone resulted in a decrease in cardiac function of 38%, as assessed by echocardiography. We also observed a 20% increase in body fat with male mice exposed to caffeine. Caffeine was dissolved in normal saline, so it was used as a control. Room air controls were used to compare to the hypoxic mice. Exposure to a single dose of caffeine during embryogenesis results in both short‐term effects on cardiac development and long‐term effects on cardiac function.—Wendler, C. C., Busovsky‐McNeal, M., Ghatpande, S., Kalinowski, A., Russell, K. S., Rivkees, S. A. Embryonic caffeine exposure induces adverse effects in adulthood. FASEB J . 23, 1272–1278 (2009)