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Macrophage migration inhibitory factor is critical to interleukin‐5‐driven eosinophilopoiesis and tissue eosinophilia triggered by Schistosoma mansoni infection
Author(s) -
Magalhaes Elizabeth S.,
Paiva Claudia N.,
Souza Heitor S. P.,
Pyrrho Alexandre S.,
Mourao-Sá Diego,
Figueiredo Rodrigo T.,
Vieira-de-Abreu Adriana,
Dutra Helio S.,
Silveira Mariana S.,
Gaspar-Elsas Maria Ignez C.,
Xavier-Elsas Pedro,
Bozza Patrícia T.,
Bozza Marcelo T.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.08-124248
Subject(s) - macrophage migration inhibitory factor , eosinophilia , schistosoma mansoni , eosinophil , immunology , biology , macrophage , interleukin 5 , interleukin , cytokine , schistosomiasis , asthma , helminths , in vitro , biochemistry
Macrophage migration inhibitory factor (MIF) participates in the pathogenesis of inflammatory diseases, including asthma, in which it enhances airway hypersensitivity and tissue eosinophilia. Herein, we investigated the role of MIF in eosinophilopoiesis and tissue eosinophilia using Schistosoma mansoni infection. MIF‐deficient ( Mif −/− ) mice had similar numbers of adult worms, eggs, and granulomas compared to wildtype mice, but the size of granulomas was strikingly reduced due to smaller numbers of eosinophils. MIF did not affect the acquired response to infection, as Mif −/− mice produced normal amounts of Th2 cytokines and IgE. Nevertheless, recombinant MIF (rMIF) behaved as a chemoattractant for eosinophils, what could partially explain the reduced eosinophilia in infected Mif −/− mice. Moreover, the percentage of eosinophils was reduced in bone marrows of Mif −/− mice chronically infected with S. mansoni compared to wild type. Mif −/− had impaired eosinophilopoiesis in response to interleukin (IL)‐5 and addition of rMIF to bone marrow cultures from IL‐5 transgenic mice enhanced the generation of eosinophils. In the absence of MIF, eosinophil precursors were unable to survive the IL‐5‐supplemented cell culture, and were ingested by macrophages. Treatment with pancaspase inhibitor z‐VAD or rMIF promoted the survival of eosinophil progenitors. Together, these results indicate that MIF participates in IL‐5‐driven maturation of eosinophils and in tissue eosinophilia associated with S. mansoni infection.—Magalhaes, E. S., Paiva, C. N., Souza, H. S. P., Pyrrho, A. S., Mourao‐Sa, D., Figueiredo, R. T., Vieira‐de‐Abreu, A., Dutra, H. S., Silveira, M. S., Gaspar‐Elsas, M. I. C., Xavier‐Elsas, P., Bozza, P. T., Bozza, M. T. Macrophage migration inhibitory factor is critical to interleukin‐5‐driven eosinophilopoiesis and tissue eosinophilia triggered by Schistosoma mansoni infection. FASEB J . 23, 1262–1271 (2009)