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Generation of transplantable, functional satellite‐like cells from mouse embryonic stem cells
Author(s) -
Chang Hsi,
Yoshimoto Momoko,
Umeda Katsutsugu,
Iwasa Toru,
Mizuno Yuta,
Fukada So-ichiro,
Yamamoto Hiroshi,
Motohashi Norio,
Miyagoe-Suzuki Yuko,
Takeda Shin'ichi,
Heike Toshio,
Nakahata Tatsutoshi
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.08-123661
Subject(s) - embryonic stem cell , stem cell , p19 cell , embryoid body , transplantation , microbiology and biotechnology , skeletal muscle , biology , matrigel , myocyte , satellite , amniotic epithelial cells , adult stem cell , immunology , in vitro , medicine , anatomy , biochemistry , aerospace engineering , gene , engineering
Satellite cells are myogenic stem cells responsible for the postnatal regeneration of skeletal muscle. Here we report the successful in vitro induction of Pax7‐positive satellite‐like cells from mouse embryonic stem (mES) cells. Embryoid bodies were generated from mES cells and cultured on Matrigel‐coated dishes with Dulbecco's modified Eagle medium containing fetal bovine serum and horse serum. Pax7‐positive satellite‐like cells were enriched by fluorescence‐activated cell sorting using a novel anti‐satellite cell antibody, SM/C‐2.6. SM/C‐2.6‐positive cells efficiently differentiate into skeletal muscle fibers both in vitro and in vivo . Furthermore, the cells demonstrate satellite cell characteristics such as extensive self‐renewal capacity in subsequent muscle injury model, long‐term engraft‐ment up to 24 wk, and the ability to be secondarily transplanted with remarkably high engraftment efficiency compared to myoblast transplantation. This is the first report of transplantable, functional satellite‐like cells derived from mES cells and will provide a foundation for new therapies for degenerative muscle disor‐ders.—Chang, H.,Yoshimoto, M., Umeda, K., Iwasa, T., Mizuno, Y., Fukada, S., Yamamoto, H., Motohashi, N., Yuko‐Miyagoe‐Suzuki, Takeda, S., Heike, T., Nakahata, T. Generation of transplantable, functional satellite‐like cells from mouse embryonic stem cells. FASEB J. 23, 1907–1919 (2009)