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The long lifespan of two bat species is correlated with resistance to protein oxidation and enhanced protein homeostasis
Author(s) -
Salmon Adam B.,
Leonard Shanique,
Masamsetti Venkata,
Pierce Anson,
Podlutsky Andrej J.,
Podlutskaya Natalia,
Richardson Arlan,
Austad Steven N.,
Chaudhuri Asish R.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.08-122523
Subject(s) - protein carbonylation , proteasome , biology , homeostasis , in vivo , oxidative stress , microbiology and biotechnology , protein aggregation , chemistry , biochemistry , oxidative damage , genetics
Altered structure, and hence function, of cellular macromolecules caused by oxidation can contribute to loss of physiological function with age. Here, we tested whether the lifespan of bats, which generally live far longer than predicted by their size, could be explained by reduced protein damage relative to short‐lived mice. We show significantly lower protein oxidation (carbonylation) in Mexican free‐tailed bats ( Tadarida brasiliensis) relative to mice, and a trend for lower oxidation in samples from cave myotis bats ( Myotis velifer ) relative to mice. Both species of bat show in vivo and in vitro resistance to protein oxidation under conditions of acute oxidative stress. These bat species also show low levels of protein ubiquitination in total protein lysates along with reduced proteasome activity, suggesting diminished protein damage and removal in bats. Lastly, we show that bat‐derived protein fractions are resistant to urea‐induced protein unfolding relative to the level of unfolding detected in fractions from mice. Together, these data suggest that long lifespan in some bat species might be regulated by very efficient maintenance of protein homeostasis.—Salmon, A. B., Leonard, S., Masamsetti, V., Pierce, A., Podlutsky, A. J., Podlutskaya, N., Richardson, A., Austad, S. N., Chaudhuri, A. R. The long lifespan of two bat species is correlated with resistance to protein oxidation and enhanced protein homeostasis. FASEB J. 23, 2317–2326 (2009)