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The oxidized phospholipids linked to human apolipoprotein(a) do not derive from circulating low‐density lipoproteins and are probably of cellular origin
Author(s) -
Edelstein Celina,
Philips Binu,
Pfaffinger Ditta,
Scanu Angelo M.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.08-122002
Subject(s) - apolipoprotein b , lipoprotein , chemistry , low density lipoprotein , lipoprotein(a) , population , recombinant dna , human plasma , biochemistry , monoclonal antibody , antibody , microbiology and biotechnology , biology , genetics , cholesterol , chromatography , gene , demography , sociology
Lipoprotein (a) [Lp(a)], a cardiovascular risk factor, is a low‐density lipoprotein (LDL) variant shown to bind to oxidized phospholipids (oxPLs); however, its binding mode and origin have not been clearly established. We isolated both LDL and Lp(a) from the plasma of a population of high‐Lp(a) subjects and in each Lp(a) particle separated apolipoprotein(a) [apo(a)], from the LDL component, Lp(a − ). These products were assayed by an ELISA using monoclonal antibody T15 with a known specificity for oxPLs. In each subject, the T15 reactivity was confined to apo(a). Moreover, the amount of oxPL bound to apo(a) was unaffected by plasma Lp(a) levels and apo(a) size polymorphism. We have previously shown that kringle V (KV) is the site of oxPL linkage in human apo(a). In this work, we expressed in human embryonic kidney cells a KV‐containing recombinant that, when purified from the medium, contained oxPLs. In summary, in human plasma Lp(a), the oxPLs are located in apo(a) and not in the circulating LDLs, suggesting a cellular origin. This latter concept is supported by the studies in which an expressed KV‐containing apo(a) microdomain exhibited oxPL reactivity. Thus, apo(a) can undergo potentially pathogenic posttranslational modifications in a cellular environment able to generate oxPL.— Edelstein, C., Philips, B., Pfaffinger, D., Scanu, A. M. The oxidized phospholipids linked to human apolipoprotein(a) do not derive from circulating low‐density lipoproteins and are probably of cellular origin. FASEB J. 23, 950–956 (2009)