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A novel stem loop control element‐dependent UGA read‐through system without translational selenocysteine incorporation in Drosophila
Author(s) -
HirosawaTakamori Mitsuko,
Ossipov Dmitri,
Novoselov Sergey V.,
Turanov Anton A.,
Zhang Yan,
Gladyshev Vadim N.,
Krol Alain,
Vorbmggen Gerd,
Jackle Herbert
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.08-116640
Subject(s) - selenoprotein , selenocysteine , biology , stop codon , stem loop , untranslated region , translation (biology) , selenoprotein p , microbiology and biotechnology , genetics , messenger rna , gene , biochemistry , glutathione peroxidase , cysteine , enzyme , glutathione
Translational read‐through of the UGA stop codon is an evolutionarily conserved feature that most prominently represents the basis of selenoprotein biosynthesis. It requires a specific cis‐acting stem loop control element, termed SECIS, which is located in the 3′‐untranslated region of eukaryotic selenoprotein mRNAs. In a search for novel factors underlying the SECIS‐directed UGA read‐through process, we identi‐fied an evolutionary conserved GTPase‐activating protein, termed GAPsec. We show that the activity of the Drosophila GAPsec (dGAPsec) is necessary to support SECIS‐dependent UGA read‐through activity in flies and the mouse homolog mGAPsec in mice tissue culture cells. However, selenoprotein biosynthesis is not impaired in flies that lack dGAPsec activity. The results indicate that GAPsec is part of a novel SECIS‐dependent translational read‐through system that does not involve selenocysteine incorporation.—Hirosawa‐Takamori, M., Ossipov, D., Novoselov, S.V., Turanov, A.A., Zhang, Y., Gladyshev, V.N., Krol, A., Vorbruggen, G., Jackle, H. A novel stem loop control element‐dependent UGA read‐through system without translational selenocysteine in‐corporation in Drosophila. FASEB J. 23, 107‐113 (2009)