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The histone subcode: poly(ADP‐ribose) polymerase‐1 (Parp‐1) and Parp‐2 control cell differentiation by regulating the transcriptional intermediary factor TIF1β and the heterochromatin protein HPlα
Author(s) -
Quénet Delphine,
Gasser Véronique,
Fouillen Laetitia,
Cammas Florence,
SanglierCianferani Sarah,
Losson Régine,
Dantzer Francoise
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.08-113464
Subject(s) - heterochromatin , poly adp ribose polymerase , biology , heterochromatin protein 1 , histone , chromatin , microbiology and biotechnology , chromatin immunoprecipitation , polymerase , genetics , gene expression , promoter , dna , gene
Recent advances reveal emerging unique functions of poly(ADP‐ribose) polymerase‐1 (Parp‐1) and Parp‐2 in heterochromatin integrity and cell differentiation. However, the chromatin‐mediated molecular and cellular events involved remain elusive. Here we describe specific physical and functional interactions of Parp‐1 and Parp‐2 with the transcriptional intermediary factor (TlF1β) and the heterochromatin proteins (HP1) that affect endodermal differentiation. We show that Parp‐2 binds to TlF1β with high affinity both directly and through HP1α. Both partners colocalize at pericentric heterochromatin in primitive endoderm‐like cells. Parp‐2 also binds to HP1β but not to HP1γ. In contrast Parp‐1 binds weakly to TlF1β and HP1β only. Both Parps selectively poly(ADP‐ribosyl)ate HP1α. Using shRNA approaches, we provide evidence for distinct participation of both Parps in endodermal differentiation. Whereas Parp‐2 and its activity are required for the relocation of TlF1β to heterochromatic foci during primitive endodermal differentiation, Parp‐1 and its activity modulate TlF1β‐HP1α association with consequences on parietal endodermal differentiation. Both Parps control TlF1β transcriptional activity. In addition, this work identifies both Parps as new modulators of the HP1‐mediated subcode histone.— Quénet, D., Gasser, V., Fouillen, L., Cammas, F., Sanglier‐Cianferani, S., Losson, R., Dantzer, F. The histone subcode: poly(ADP‐ribose) polymerase‐1 (Parp‐1) and Parp‐2 control cell differentiation by regulating the transcriptional intermediary factor TIF1β and the heterochromatin protein HPlα. FASEB J. 22, 3853–3865 (2008)