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Initial evaluation of the use of USPIO cell labeling and noninvasive MR monitoring of human tissue‐engineered vascular grafts in vivo
Author(s) -
Nelson G. N.,
Roh J. D.,
Mirensky T. L.,
Wang Y.,
Yi T.,
Tellides G.,
Pober J. S.,
Shkarin P.,
Shapiro E. M.,
Saltzman W. M.,
Papademetris X.,
Fahmy T. M.,
Breuer C. K.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.08-107367
Subject(s) - in vivo , ex vivo , animal model , chemistry , medicine , pathology , biomedical engineering , radiology , biology , microbiology and biotechnology
This pilot study examines noninvasive MR monitoring of tissue‐engineered vascular grafts (TEVGs) in vivo using cells labeled with iron oxide nanoparticles. Human aortic smooth muscle cells (hASMCs) were labeled with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles. The labeled hASMCs, along with human aortic endothelial cells, were incorporated into eight TEVGs and were then surgically implanted as aortic interposition grafts in a C.B–17 SCID/bg mouse host. USPIO‐labeled hASMCs persisted in the grafts throughouta3wk observation period and allowed noninvasive MR imaging of the human TEVGs for real‐time, serial monitoring of hASMC retention. This study demonstrates the feasibility of applying noninvasive imaging techniques for evaluation of in vivo TEVG performance.— Nelson, G. N., Roh, J. D., Mirensky, T. L., Wang, Y., Yi, T., Tellides, G., Pober, J. S., Shkarin, P., Shapiro, E. M., Saltzman, W. M., Papademetris, X., Fahmy, T. M., Breuer, C. K. Initial evaluation of the use of USPIO cell labeling and noninvasive MR monitoring of human tissue‐engineered vascular grafts in vivo . FASEB J. 22, 3888–3895 (2008)