Premium
The immunoregulatory glycan‐binding protein galectin‐1 triggers human platelet activation
Author(s) -
Pacienza Natalia,
Pozner Roberto G.,
Bianco Germáin A.,
D'Atri Lina P.,
Croci Diego O.,
Negrotto Soledad,
Malaver Elisa,
Gómez Ricardo M.,
Rabinovich Gabriel A.,
Schattner Mirta
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.07-9524com
Subject(s) - platelet , platelet activation , microvesicles , microbiology and biotechnology , chemistry , galectin , thrombin , galectin 3 , glycan , inflammation , endogeny , lectin , biochemistry , biology , immunology , glycoprotein , microrna , gene
Platelet activation is a critical process during inflammation, thrombosis, and cancer. Here, we show that galectin‐1, an endogenous lectin with immunoregulatory properties, plays a key role in human platelet activation and function. Galectin‐1 binds to human platelets in a carbohydrate‐dependent manner and synergizes with ADP or thrombin to induce platelet aggregation and ATP release. Furthermore, galectin‐1 induces F‐actin polymerization, up‐regulation of P‐se‐lectin, and GPIIIa expression; promotes shedding of microvesicles; and triggers conformational changes in GPIIb/IIIa. In addition, exposure to this lectin favors the generation of leukocyte‐platelet aggregates. A further mechanistic analysis revealed the involvement of Ca 2+ and cyclic nucleotide‐dependent pathways in ga‐lectin‐1‐mediated control of platelet activation. Finally, expression of endogenous galectin‐1 in human platelets contributes to ADP‐induced aggregation. Our study reveals a novel unrecognized role for galectin‐1 in the control of platelet physiology with potential implications in thrombosis, inflammation, and metastasis. Pacienza N., Pozner, R G., Bianco, G. A., D'Atri L. P., Croci, D. O., Negrotto, S., Malaver, E., Gómez R. M., Rabinovich, G. A., Schattner M. The immunoregula‐tory glycan‐binding protein galectin‐1 triggers human platelet activation. FASEB J. 22, 1113–1123 (2008)