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Dendritic cells support angiogenesis and promote lesion growth in a murine model of endometriosis
Author(s) -
Fainaru Ofer,
Adini Avner,
Benny Ofra,
Adini Irit,
Short Sarah,
Bazinet Lauren,
Nakai Kei,
Pravda Elke,
Hornstein Mark D.,
D'Amato Robert J.,
Folkman Judah
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.07-9034com
Subject(s) - angiogenesis , endometriosis , medicine , stromal cell , cancer research , pathology , lewis lung carcinoma , peritoneal cavity , immunology , cancer , metastasis , anatomy
Endometriosis affects 10–15% of women and is associated with pelvic pain and infertility. Angiogenesis plays an essential role in its pathogenesis. Dendritic cells (DCs) were recently implicated in supporting tumor angiogenesis. As both tumors and endometriosis lesions depend on angiogenesis, we investigated the possibility that DCs may also play a role in endometriosis. We induced endometriosis in 8‐wk‐old female C57BL/6 mice by implantation of autologous endometrium into the peritoneal cavity. We observed an abundance of CD11c + DCs infiltrating sites of angiogenesis in endometriosis lesions. We noticed a similar pattern of infiltrating DCs at sites of angiogenesis in the peritoneal Lewis lung carcinoma tumor model. These DCs were immature (major histocompatability complex class II low ) and expressed vascular endothelial growth factor receptor 2. Peritoneal implanted bone marrow‐derived DCs (BMDCs) incorporated into both endometriosis lesions and into B16 melanoma tumors and enhanced their growth at 8 days compared with controls (5.1 ±2.5 vs. 1.5±0.5mm 2 , n =4 and 4, P <0.0001 for endometriosis;67.6±15.1 vs. 22.7±14.6 mm 2 , n=5 and 7, P=0.0004 for mouse melanoma). Finally, immature BMDCs but not mature BMDCs enhanced microvascular endothelial cell migration in vitro (219±51 vs. 93±32 cells, P =0.02). Based on these findings, we suggest a novel role for DCs in supporting angiogenesis and promoting lesion growth both in endometriosis and in tumors.—Fainaru, O., Adini, A., Benny, O., Adini, I., Short, S., Bazinet, L., Nakai, K., Pravda, E., Hornstein, M. D., D'Amato, R. J., Folkman, J. Dendritic cells support angiogenesis and promote lesion growth in a murine model of endometriosis. FASEB J. 22, 522−529 (2008)