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Expression, transport, and axonal sorting of neuronal CCL21 in large dense‐core vesicles
Author(s) -
Jong Eiko K.,
Vinet Jonathan,
Stanulovic Vesna S.,
Meijer Michel,
Wesseling Evelyn,
Sjollema Klaas,
Boddeke Hendrikus W. G. M.,
Biber Knut
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.07-101907
Subject(s) - microglia , neuron , neuroscience , microbiology and biotechnology , biology , inflammation , immunology
Neurons are highly polarized cells, and neuron‐neuron communication is based on directed transport and release of neurotransmitters, neuropeptides, and neurotrophins. Directed communication may also be attributed to neuron‐microglia signaling, since neuronal damage can induce a microglia reaction at specific sites only. However, the mechanism underlying this site‐specific microglia reaction is not yet under stood. Neuronal CCL21 is a microglia‐activating chemo kine, which in brain is solely found in endangered neurons and is therefore a candidate for neuronmicroglia signaling. Here we present that neuronal CCL21 is sorted into large dense‐core vesicles, the secretory granules of the regulated release pathway of neurons. Live‐cell imaging studies show preferential sorting of CCL21‐containing vesicles into axons, indi cating its directed transport. Thus, mouse neurons express and transport a microglia activating factor very similar to signaling molecules used in neuron‐neuron communication. These data show for the first time the directed transport of a microglia activating factor in neurons and corroborate the function of neuronal CCL21 in directed neuron‐microglia communication.— De Jong, E. K., Vinet, J., Stanulovic, V. S., Meijer, M., Wesseling, E., Sjollema, K., Boddeke, H. W. G. M., Biber, K. Expression, transport, and axonal sorting of neuronal CCL21 in large dense‐core vesicles. FASEB J. 22, 4136–4145 (2008)