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Glucose and leptin induce apoptosis in human β‐ cells and impair glucose‐stimulated insulin secretion through activation of c‐Jun N‐terminal kinases
Author(s) -
Maedler Kathrin,
Schulthess Fabienne T.,
Bielman Christelle,
Berney Thierry,
Bonny Christophe,
Prentki Marc,
Donath Marc Y.,
Roduit Raphael
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.07-101824
Subject(s) - medicine , endocrinology , leptin , kinase , beta cell , pancreatic islets , insulin , secretion , apoptosis , c jun , insulin resistance , islet , biology , chemistry , microbiology and biotechnology , biochemistry , transcription factor , gene , obesity
c‐Jun N‐terminal kinases (SAPK/JNKs) are activated by inflammatory cytokines, and JNK sig naling is involved in insulin resistance and β‐ cell secre tory function and survival. Chronic high glucose con centrations and leptin induce interleukin‐1 Vβ (IL‐1β) secretion from pancreatic islets, an event that is possi bly causal in promoting β‐ cell dysfunction and death. The present study provides evidence that chronically elevated concentrations of leptin and glucose induce P‐cell apoptosis through activation of the JNK pathway in human islets and in insulinoma (INS 832/13) cells. JNK inhibition by the dominant inhibitor JNK‐binding domain of IB1/JIP‐1 (JNKi) reduced JNK activity and apoptosis induced by leptin and glucose. Exposure of human islets to leptin and high glucose concentrations leads to a decrease of glucose‐induced insulin secretion, which was partly restored by JNKi. We detected an interplay between the JNK cascade and the caspase 1/IL‐1 β‐ converting enzyme in human islets. The caspase 1 gene, which contains a potential activating protein‐1 binding site, was up‐regulated in pancreatic sections and in isolated islets from type 2 diabetic patients. Similarly, cultured human islets exposed to high glucose‐ and leptin‐induced caspase 1 and JNK inhibition prevented this up‐regulation. Therefore, JNK inhibition may protect β‐ cells from the deleterious effects of high glucose and leptin in diabetes.— Maedler, K., Schulthess, F. T., Bielman, C., Berney, T., Bonny, C., Prentki, M., Donath, M. Y., Roduit, R. Glucose and leptin induce apoptosis in human β‐ cells and impair glucose‐stimulated insulin secretion through activation of c‐Jun N‐terminal kinases. FASEB J. 22, 1905–1913 (2008)