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A genome‐wide survey suggests an osmoprotective role for vacuolar Ca 2+ release in cell wall‐compromised yeast
Author(s) -
Loukin Stephen,
Zhou Xinliang,
Kung Ching,
Saimi Yoshiro
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.07-101410
Subject(s) - mechanosensitive channels , calcineurin , calcium , yeast , cell wall , osmotic shock , microbiology and biotechnology , saccharomyces cerevisiae , patch clamp , biology , biophysics , chemistry , gene , biochemistry , ion channel , receptor , medicine , surgery , organic chemistry , transplantation
In yeast, osmotic upshock causes are lease of vacuolar Ca 2+ through the mechanosensitive transient receptor potential channel, Yvc1. We screened the collection of 4810 yeast gene deletants twice for alterations in this response in an attempt to find elements that regulate the amount of vacuolar Ca 2+ or the Yvc1 channel. Severe overresponders and underresponders to upshock were further scrutinized for their calcium content with 45 Ca and their Yvcl electrophysiological activities under patch‐clamp. The severe underresponders have lower calcium content but no change in Yvcl activity. The strong overresponders, most of which are deleted of genes involved in cell wall metabolism, have higher calcium content. Wall mutations are known to up‐regulate Ca 2+ ‐calcineurin‐dependent genes. It appears that stress on the cell wall induces Ca 2+ accumulation, adaptively anticipating the need in defense or repair against future stress, including osmotic stress.—Loukin, S., Zhou, X., Kung, C., Saimi, Y. A genome‐wide survey suggests an osmoprotective role for vacuolar Ca 2+ release in cell wall‐compromised yeast. FASEB J. 22, 2405–2415 (2008)