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Integrated approach to designing growth factor delivery systems
Author(s) -
Chen Ruth R.,
Silva Eduardo A.,
Yuen William W.,
Brock Andrea A.,
Fischbach Claudia,
Lin Angela S.,
Guldberg Robert E.,
Mooney David J.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.06-7873com
Subject(s) - angiogenesis , vascular endothelial growth factor , growth factor , context (archaeology) , therapeutic angiogenesis , tissue engineering , medicine , bench to bedside , perfusion , ischemia , delivery system , bioinformatics , vegf receptors , biomedical engineering , neovascularization , biology , cancer research , medical physics , receptor , paleontology
Growth factors have been widely used in strategies to regenerate and repair diseased tissues, but current therapies that go directly from bench to bedside have had limited clinical success. We hypothesize that engineering successful therapies with recombinant proteins will often require specific quantitative information of the spatiotemporal role of the factors and the development of sophisticated delivery approaches that provide appropriate tissue exposures. This hypothesis was tested in the context of therapeutic angiogenesis. An in vitro model of angiogenesis was adapted to quantify the role of the concentration/gradient of vascular endothelial growth factor [VEGF (165)] on microvascular endothelial cells, and a delivery system was then designed, based on a mathematical model, to provide the desired profile in ischemic mice hindlimbs. This system significantly enhanced blood vessel formation, and perfusion and recovery from severe ischemia. This general approach may be broadly applicable to growth factor therapies.— Chen R. R., Silva, E. A., Yuen, W. W., Brock, A. A., Fischbach, C., Lin, A. S., Guldberg, R. E., Mooney D. J. Integrated approach to designing growth factor delivery systems. FASEB J . 21, 3896–3903 (2007)