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Astroglia‐derived retinoic acid is a key factor in glia‐induced neurogenesis
Author(s) -
Környei Z.,
Gócza E.,
Rühl R.,
Orsolits B.,
Vörös E.,
Szabó B.,
Vágovits B.,
Madarász E.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.06-7756com
Subject(s) - retinoic acid , neurogenesis , microbiology and biotechnology , biology , retinoic acid receptor , neuron , cellular differentiation , neuroscience , biochemistry , cell culture , genetics , gene
Astroglial cells are essential components of the neurogenic niches within the central nervous system. Emerging evidence suggests that they are among the key regulators of postnatal neurogenesis. Although astrocytes have been demonstrated to possess the potential to instruct stem cells to adopt a neuronal fate, little is known about the nature of the glia‐derived instructive signals. Here we propose that all‐trans reti‐noic acid, one of the most powerful morphogenic molecules regulating neuronal cell fate commitment, may be one of the glia‐derived factors directing astro‐glia‐induced neurogenesis. According to data obtained from several complementary approaches, we show that cultured astrocytes express the key enzyme mRNAs of retinoic acid biosynthesis and actively produce all‐trans retinoic acid. We show that blockage of retinoic acid signaling by the pan‐RAR antagonist AGN193109 prevents glia‐induced neuron formation by noncommitted stem cells. Therefore, we provide strong in vitro evidence for retinoic acid action in astroglia‐induced neuronal differentiation.—Környei, Z., Gócza, E., Rühl, R., Vörös, E., Orsolits, B., Szabo, B., Vágovits, B., Madarász, E. Astroglia‐derived retinoic acid is a key factor in glia‐induced neurogenesis. FASEB J. 21, 2496–2509 (2007)