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Femtosecond laser: a new intradermal DNA delivery method for efficient, long‐term gene expression and genetic immunization
Author(s) -
Zeira Evelyne,
Manevitch Alexandra,
Manevitch Zakharia,
Kedar Eli,
Gropp Michal,
Daudi Nili,
Barsuk Rimma,
Harati Menahem,
Yotvat Hagit,
Troilo Philip J.,
Griffiths Thomas G.,
Pacchione Stephen J.,
Roden Dana F.,
Niu Zhutian,
Nussbaum Ofer,
Zamir Gideon,
Papo Orit,
Hemo Izhack,
Lewis Aaron,
Galun Eithan
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.06-7528com
Subject(s) - hbsag , transduction (biophysics) , gene delivery , dna vaccination , genetic enhancement , plasmid , electroporation , transfection , virology , antigen , immunization , vaccination , hepatitis b virus , biology , microbiology and biotechnology , gene expression , gene , immunology , virus , genetics , biochemistry
ABSTRACT A femtosecond laser beam gene transduction (SG‐LBGT) system is described as a novel and efficient method of intradermal (i.d.) nonviral gene delivery in mice by permeabilizing cells utilizing femtosecond laser pulses. Using this approach, significant gene expression and efficient dermal transduction lasting for >7 months were obtained. The ability of this new DNA gene transfer method to enhance genetic vaccination was tested in BALB/C mice. A single i.d. injection of a plasmid (10 μg) containing the hepatitis B virus (HBV) surface antigen (HBsAg), followed by pulses of laser, induced high titers of HBsAg‐specific antibodies lasting for >210 days and increased levels of IgG1, IgG2a, IFNγ, and IL‐4, indicating the activation of both Th1 and Th2 cells. Moreover, mice vaccinated using the SG‐LBGT followed by challenge with pHBV showed increased protection against viral challenge, as detected by decreased levels of HBV DNA, suggesting an efficient Th1 effect against HBV‐infected replicating cells. Tumor growth retardation was induced in vacci‐nated mice challenged with an HBsAg‐expressing syn‐geneic tumor. In most of the parameters tested, administration of plasmid followed by laser application was significantly more effective and prolonged than that of plasmid alone. Tissue damage was not detected and integration of the plasmid into the host genomic DNA probably did not occur. We suggest that the LBGT method is an efficient and safe technology for in vivo gene expression and vaccination and emphasizes its potential therapeutic applications for i.d. nonviral gene delivery.—Zeira, E., Manevitch, A., Manevitch, Z., Kedar, E., Gropp, M., Daudi, N., Barsuk, R., Harati, M., Yotvat, H., Troilo, P. J., Griffiths, T. G., II, Pacchione, S. J., Roden, D. F., Niu, Z., Nussbaum, O., Zamir, G., Papo, O., Hemo, I., Lewis, A., Galun, E. Femtosecond laser: a new intradermal DNA delivery method for efficient, long‐term gene expression and genetic immunization. FASEB J. 21, 3522–3533 (2007)