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Regulation of capacitative calcium entries by α1‐syntrophin: association of TRPC1 with dystrophin complex and the PDZ domain of α1‐syntrophin
Author(s) -
Vandebrouck Aurélie,
Sabourin Jessica,
Rivet Jéröme,
Balghi Haouria,
Sebille Stéphane,
Kitzis Alain,
Raymond Guy,
Cognard Christian,
Bourmeyster Nicolas,
Constantin Bruno
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.06-6683com
Subject(s) - trpc1 , dystrophin , pdz domain , microbiology and biotechnology , chemistry , skeletal muscle , voltage dependent calcium channel , calcium , immunoprecipitation , biology , transient receptor potential channel , biochemistry , anatomy , receptor , organic chemistry , gene
Calcium mishandling in Duchenne dystrophic muscle suggested that dystrophin, a membrane‐associated cytoskeleton protein, might regulate calcium signaling cascade such as calcium influx pathway. It was previously shown that abnormal calcium entries involve uncontrolled stretch‐activated currents and store‐operated Ca 2+ currents supported by TRPC1 channels. Moreover, our recent work demonstrated that reintroduction of minidystrophin in dystrophic myotubes restores normal capacitative calcium entries (CCEs). However, until now, no molecular link between the dystrophin complex and calcium entry channels has been described. This study is the first to show by coimmunoprecipitation assays the molecular association of TRPC1 with dystrophin and α1‐syntrophin in muscle cells. TRPC1 was also associated with α1‐syntrophin in dystrophic muscle cells independently of dystrophin. Furthermore, glutathione S ‐transferase (GST) pull‐down assays showed that TRPC1 binds to the α1‐syntrophin PDZ domain. Transfected recombinant α1‐syntrophin formed a complex with TRPC1 channels and restored normal CCEs in dystrophic muscle cells. We suggest that normal regulation of CCEs in skeletal muscle depends on the association between TRPC1 channels and α1‐syntrophin that may anchor the store‐operated channels to the dystrophin‐associated protein complex (DAPC). The loss of this molecular association could participate in the calcium alterations observed in dystrophic muscle cells. This study provides a new model for the regulation of calcium influx by interaction with the scaffold of the DAPC in muscle cells.—Vandebrouck, A., Sabourin, J., Rivet, J., Balghi, H., Sebille, S., Kitzis, A., Raymond, G., Cognard, C., Bourmeyster, N., Constantin, B. Regulation of capacitative calcium entries by α1‐syntrophin: association of TRPC1 with dystrophin complex and the PDZ domain of α1‐syntrophin. FASEB J. 21, 608–617 (2007)