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19 F magnetic resonance imaging for stem/progenitor cell tracking with multiple unique perfluorocarbon nanobeacons
Author(s) -
Partlow Kathryn C.,
Chen Junjie,
Brant Jason A.,
Neubauer Anne M.,
Meyerrose Todd E.,
Creer Michael H.,
Nolta Jan A.,
Caruthers Shelton D.,
Lanza Gregory M.,
Wicldine Samuel A.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.06-6505com
Subject(s) - progenitor cell , in vivo , stem cell , chemistry , cd34 , cell , biophysics , microbiology and biotechnology , biology , biochemistry
MRI has been employed to elucidate the migratory behavior of stem/progenitor cells noninva‐sively in vivo with traditional proton ( 1 H) imaging of iron oxide nanoparticle‐labeled cells. Alternatively, we demonstrate that fluorine ( 19 F) MRI of cells labeled with different types of liquid perfluorocarbon (PFC) nanoparticles produces unique and sensitive cell markers distinct from any tissue background signal. To define the utility for cell tracking, mononuclear cells harvested from human umbilical cord blood were grown under proendothelial conditions and labeled with nanoparticles composed of two distinct PFC cores (perfluorooctylbromide and perfluoro‐15‐crown‐5 ether). The sensitivity for detecting and imaging labeled cells was defined on 11.7T (research) and 1.5T (clinical) scanners. Stem/progenitor cells (CD34 + CD133 + CD31 + ) readily internalized PFC nanoparticles without aid of adjunctive labeling techniques, and cells remained functional in vivo. PFC‐labeled cells exhibited distinct 19 F signals and were readily detected after both local and intravenous injection. PFC nanoparticles provide an unequivocal and unique signature for stem/progenitor cells, enable spatial cell localization with 19 F MRI, and permit quantification and detection of multiple fluorine signatures via 19 F MR spectroscopy. This method should facilitate longitudinal investigation of cellular events in vivo for multiple cell types simultaneously.—Partlow, K. C., Chen, J., Brant, J. A., Neubauer, A. M., Meyerrose, T. E., Creer, M. H., Nolta, J. A., Caruthers, S. D., Lanza, G. M., Wickline, S. A. 19F magnetic resonance imaging for stem/progenitor cell tracking with multiple unique perfluorocarbon nanobeacons. FASEB J. 21, 1647–1654 (2007)