Premium
Isolation of a mesenchymal cell population from murine dermis that contains progenitors of multiple cell lineages
Author(s) -
Crigler Lauren,
Kazhanie Amita,
Yoon Tae-Jin,
Zakhari Julia,
Anders Joanna,
Taylor Barbara,
Virador Victoria M.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.06-5880com
Subject(s) - mesenchymal stem cell , biology , dermis , progenitor cell , population , microbiology and biotechnology , hair follicle , keratinocyte , stem cell , epidermis (zoology) , pathology , anatomy , cell culture , medicine , genetics , environmental health
The skin contains two known subpopula–tions of stem cells/epidermal progenitors: a basal kera–tinocyte population found in the interfollicular epithelium and cells residing in the bulge region of the hair follicle. The major role of the interfollicular basal kera–tinocyte population may be epidermal renewal, whereas the bulge population may only be activated and recruited to form a cutaneous epithelium in case of trauma. Using 3–dimensional cultures of murine skin under stress conditions in which only reserve epithelial cells would be expected to survive and expand, we demonstrate that a mesenchymal population resident in neonatal murine dermis has the unique potential to develop an epidermis in vitro. In monolayer culture, this dermal subpopulation has long–term survival capabilities in restricted serum and an inducible capacity to evolve into multiple cell lineages, both epithelial and mesenchymal, depending on culture conditions. When grafted subcutaneously, this dermal subpopulation gave rise to fusiform structures, reminiscent of disorganized muscle, that stained positive for smooth muscle actin and desmin; on typical epidermal grafts, abundant melanocytes appeared throughout the dermis that were not associated with hair follicles. The multipotential cells can be repeatedly isolated from neonatal murine dermis by a sequence of differential centrif–ugation and selective culture conditions. These results suggest that progenitors capable of epidermal differentiation exist in the mesenchymal compartment of an abundant tissue source and may have a function in mesenchy–mal–epithelial transition upon insult. Moreover, these cells could be available in sufficient quantities for lineage determination or tissue engineering applications.–Crigler, L., Kazhanie, A., Yoon, T.‐J., Zakhari, J., Anders, J., Taylor, B., Virador, V. M. Isolation of a mesenchymal cell population from murine dermis that contains progenitors of multiple cell lineages. FASEB J. 21, 2050–2063 (2007)