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Pivotal role of integrin 0'5H1 in hypotonic stress‐ induced responses of human endothelium
Author(s) -
Hirakawa Masakazu,
Oike Masahiro,
Watanabe Michi,
Karashima Yuji,
Ito Yushi
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.05-5580com
Subject(s) - rhoa , microbiology and biotechnology , integrin , stress fiber , paxillin , tyrosine phosphorylation , focal adhesion , chemistry , phosphorylation , biology , signal transduction , biochemistry , receptor
We have previously reported that both hypotonic stress (HTS) and lysophosphatidic acid (LPA) induce ATP release and a transient reorganization of actin through sequential activation of RhoA/ Rho‐kinase and focal adhesion kinase F‐actin (FAK)/ paxillin in human umbilical cord vein endothelial cells (HUVECs). LPA is known to induce the activation of RhoA via its specific receptors, but the mechanisms by which HTS initiates these intracellular signals are not known. The present study aimed to identify the molecule(s) that are unique to the sensing and/or transducing the mechanical stress. Reverse transcriptase‐polymerase chain reaction revealed the expression of several integrin subunits in HUVECs. Anti‐integrin 51 antibody (Ab), but not anti‐integrin α2, α6, αv, or ၦ4 antibodies, inhibited HTS‐induced RhoA translocation, tyrosine phosphorylation of FAK and paxillin, ATP release, and actin reorganization. However, the LPA‐induced ATP release and actin reorganization were not inhibited by any of these anti‐integrin antibodies, indicating that integrin 51 plays a pivotal role in the HTS‐induced but not in the LPA‐induced responses. It is therefore reasonable to assume that this particular subtype of integrin is involved in the initiation of the responses induced by mechanical stimuli in HUVECs.—Hirakawa, M., Oike, M., Watanabe, M., Karashima, Y., Ito, Y. Pivotal role of integrin 51 in hypotonic stress‐induced responses of human endothelium. FASEB J. 20, 1992–1999 (2006)