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Aspirin regulates expression and function of scavenger receptor‐BI in macrophages: studies in primary human macrophages and in mice
Author(s) -
Tancevski Ivan,
Weliinger Andreas,
Schgoer Wilfried,
Eller Philipp,
Cuzzocrea Salvatore,
Foeger Bernhard,
Patsch Josef R.,
Ritsch Andreas
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.05-5368com
Subject(s) - scavenger receptor , aspirin , chemistry , in vivo , receptor , naproxen , endocrinology , medicine , ibuprofen , sodium salicylate , pharmacology , cholesterol , biology , biochemistry , lipoprotein , pathology , alternative medicine , microbiology and biotechnology
Scavenger receptor class B type I (SR‐BI) has been shown to be expressed in human atherosclerotic plaque macrophages, where it is believed to reduce atherosclerosis by promoting cholesterol efflux. In this study we investigated the influence of aspirin and other NSAIDs on SR‐BI expression and function in cultured human macrophages as well as in different mouse strains. Incubation of human macrophages with 0.5 mmol/l aspirin resulted in increased SR‐BI protein expression and increased uptake of HDL‐associated [ 3 H]cholesteryl oleate without changes of SR‐BI mRNA levels. In contrast, using 5 mmol/l of aspirin, SR‐BI expression and function were significantly decreased. Sodium salicylate exerted similar effects on SR‐BI expression, whereas no effects were observed using known COX1/2 inhibitors ibuprofen and naproxen, respectively. In in vivo studies low‐dose aspirin treatment (6 mg/kg·day) induced SR‐BI expression in wild‐type and PPAR‐α knockout mice, respectively, whereas the opposite effect was observed upon high‐dose aspirin treatment (60 mg/kg·day) in these animals. We could show that COX‐independent effects of aspirin were able to enhance expression of SR‐BI in macrophages in a post‐transcriptional, PPAR‐α independent way, suggesting a novel pharmacologic effect of aspirin.—Tancevski, I., Wehinger, A., Schgoer, W., Eller, P., Cuzzocrea, S., Foeger, B., Patsch, J. R., Ritsch, A. Aspirin regulates expression and function of scavenger receptor‐BI in macrophages: studies in primary human macrophages and in mice. FASEB J. 20, 1328–1335 (2006)