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FGF‐4 regulates neural progenitor cell proliferation and neuronal differentiation
Author(s) -
Kosaka Nobuyoshi,
Kodama Maho,
Sasaki Hideo,
Yamamoto Yusuke,
Takeshita Fumitaka,
Takahama Yasushi,
Sakamoto Hiromi,
Kato Takashi,
Terada Masaaki,
Ochiya Takahiro
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.05-5293fje
Subject(s) - neurosphere , fibroblast growth factor , subventricular zone , progenitor cell , neural stem cell , biology , microbiology and biotechnology , neurogenesis , cellular differentiation , in situ hybridization , stem cell , neuroscience , adult stem cell , messenger rna , genetics , gene , receptor
ABSTRACT The FGF‐4 (fibroblast growth factor 4, known as HST‐1) protein is an important mitogen for a variety of cell types. However, only limited information is available concerning tissue distribution and the biological role of FGF‐4 in the brain. In situ hybridization analysis revealed localization of mouse Fgf‐4 mRNA in the normal postnatal mouse hippocampus, subventricu‐lar zone (SVZ), and the rostral migratory stream where new neurons generate, migrate, and become incorporated into the functional circuitry of the brain. We also investigated whether FGF‐4 could promote both proliferation and differentiation of the neural progenitor cells by using an in vitro neurosphere assay. The addition of recombinant FGF‐4 generated large prolif‐erative spheres that have a multipotent differentiation ability. Furthermore, recombinant FGF‐4 significantly promotes neuronal differentiation in attached clonal neurosphere culture. These findings suggest that FGF‐4 has an ability to promote neural stem cell proliferation and neuronal differentiation in the postnatal brain.—Kosaka, N., Kodama, M., Sasaki, H., Yamamoto, Y., Takeshita, F., Takahama, Y., Sakamoto, H., Kato, T., Terada, M., Ochiya, T. FGF‐4 regulates neural progenitor cell proliferation and neuronal differentiation. FASEB J. 20, E623–E629 (2006)