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Vascular tenascin‐C regulates cardiac endothelial phenotype and neovascularization
Author(s) -
Ballard Victoria L. T.,
Sharma Arti,
Duignan Inga,
Holm Jacquelyne M.,
Chin Andrew,
Choi Ruby,
Hajjar Katherine A.,
Wong ShingChiu,
Edelberg Jay M.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.05-5131fje
Subject(s) - angiogenesis , tenascin c , endothelial progenitor cell , endothelial stem cell , neovascularization , biology , progenitor cell , matricellular protein , microbiology and biotechnology , cancer research , immunology , pathology , medicine , stem cell , extracellular matrix , in vitro , biochemistry
Microenvironmental cues mediate postnatal neovascularization via modulation of endothelial cell and bone marrow‐derived endothelial progenitor cell (EPC) activity. Numerous signals regulate the activity of both of these cell types in response to vascular injury, which suggests that parallel mechanisms regulate angiogenesis in the vascular beds of both the heart and bone marrow. To identify mediators of such shared pathways, in vivo bone marrow/cardiac phage display biopanning was performed and led to the identification of tenascin‐C as a candidate protein. Functionally, tenascin‐C inhibits cardiac endothelial cell spreading and enhances migration in response to angiogenic growth factors. Analysis of human coronary thrombi revealed tenascin‐C protein expression colocalized with the endothelial cell/EPC marker Tie‐2 in intrathrombi vascular channels. Immunostains in the rodent heart demonstrated that tenascin‐C also colocalizes with EPCs homing to sites of cardiac angiogenic induction. To determine the importance of tenascin‐C in cardiac neovascularization, we used an established cardiac transplantation model and showed that unlike wild‐type mice, tenascin‐C −/− mice fail to vascularize cardiac allografts. This demonstrates for the first time that tenascin‐C is essential for postnatal cardiac angiogenic function. Together, our data highlight the role of tenascin‐C as a microenvironmental regulator of cardiac endothelial/EPC activity.