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Multipotentiality, homing properties, and pyramidal neurogenesis of CNS‐derived LeX(ssea‐1) + /CXCR4 + stem cells
Author(s) -
Corti Stefania,
Locatelli Federica,
Papadimitriou Dimitra,
Donadoni Chiara,
Del Bo Roberto,
Fortunato Francesco,
Strazzer Sandra,
Salani Sabrina,
Bresolin Nereo,
Comi Giacomo P.
Publication year - 2005
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.05-4170fje
Subject(s) - homing (biology) , neural stem cell , neurogenesis , stem cell , cxcr4 , microbiology and biotechnology , population , biology , hippocampal formation , precursor cell , neuroscience , cell , immunology , medicine , immune system , biochemistry , ecology , chemokine , environmental health
Achieving efficient distribution of neural stem cells throughout the central nervous system (CNS) and robust generation of specific neurons is a major challenge for the development of cell‐mediated therapy for neurodegenerative diseases. We isolated a primitive neural stem cell subset, double positive for LeX(Le) and CXCR4(CX) antigens that possesses CNS homing potential and extensive neuronal repopulating capacity. Le + CX + cells are multipotential and can generate neurons as well as myogenic and endothelial cells. In vivo Le + CX + cells displayed widespread incorporation and differentiated into cortical and hippocampal pyramidal neurons. Since intravenous delivery could be a less invasive route of transplantation, we investigated whether Le + CX + cells could migrate across endothelial monolayers. Intracerebral coadministration of SDF enabled migration of intravenously injected Le + CX + cells into the CNS and a small, yet significant, number of donor cells differentiated into neurons. The isolation of a specific neural stem cell population could offer major advantages to neuronal replacement strategies.