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Capsaicin‐sensitive sensory neurons regulate myocardial function and gene expression pattern of rat hearts: a DNA microarray study
Author(s) -
Zvara Ágnes,
Bencsik Péter,
Fodor Gabriella,
Csont Tamás,
Hackler László,
Dux Mária,
Fürst Susanna,
Jancsó Gábor,
Puskás László G.,
Ferdinandy Péter
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.05-4060fje
Subject(s) - capsaicin , trpv1 , receptor , biology , gene expression , medicine , endocrinology , gene , transient receptor potential channel , biochemistry
We have previously shown that capsaicin‐sensitive sensory nerves contribute to the regulation of normal cardiac function and to the development of cardiac adaptation to ischemic stress; however, the underlying molecular mechanisms remain unknown. Therefore, here we assessed cardiac functional alterations and relative gene expression changes by DNA microarray analysis of 6400 genes in rat hearts 7 days after the end of systemic capsaicin treatment protocol leading to selective sensory chemodenervation. Capsaicin pretreatment resulted in a cardiac dysfunction characterized by elevation of left ventricular end‐diastolic pressure and led to altered expression of 80 genes of known function or homology to known sequences. Forty‐seven genes exhibited significant up‐regulation and 33 genes were down‐regulated (changes ranged from −3.9 to +4.8fold). The expression changes of 10 selected genes were verified, and an additional 11 genes were examined by real‐time quantitative PCR. This is the first demonstration that gene expression changes in the heart due to capsaicin pretreatment included vanilloid receptor‐1 (capsaicin receptor), transient receptor potential protein, GABA receptor rho‐3 subunit, 5hydroxytryptamine 3 receptor B, neurokinin receptor 2, endothelial nitric oxide synthase, matrix metalloproteinase‐13, cytochrome P450, farnesyl‐transferase, ApoB, and leptin. None of the genes have been previously shown to be involved in the mechanism of the cardiac functional effects of sensory chemodenervation by capsaicin. We conclude that capsaicin‐sensitive sensory nerves play a significant role in the regulation of a variety of neuronal and non‐neuronal genes in the heart and possibly in other tissues as well.