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Ahnak is critical for cardiac Ca(v)1.2 calcium channel function and its β‐adrenergic regulation
Author(s) -
Haase Hannelore,
Alvarez Julio,
Petzhold Daria,
Doller Anke,
Behlke Joachim,
Erdmann Jeanette,
Hetzer Roland,
RegitzZagrosek Vera,
Vassort Guy,
Morano Ingo
Publication year - 2005
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.05-3997com
Subject(s) - protein subunit , chemistry , peptide , adrenergic receptor , receptor , biophysics , biology , biochemistry , gene
ABSTRACT Defective L‐type Ca 2+ channel (I CaL ) regulation is one major cause for contractile dysfunction in the heart. The I CaL is enhanced by sympathetic nervous stimulation: via the activation of β‐adrenergic receptors, PKA phosphorylates the α1C(Ca V 1.2)‐ and β2‐channel subunits and ahnak, an associated 5643‐amino acid (aa) protein. In this study, we examined the role of a naturally occurring, genetic variant Ile5236Thr‐ahnak on I CaL . Binding experiments with ahnak fragments (wild‐type, Ile5236Thr mutated) and patch clamp recordings revealed that Ile5236Thr‐ahnak critically affected both β2 subunit interaction and I CaL regulation. Binding affinity between ahnak‐C1 (aa 4646‐5288) and β2 subunit decreased by ≈50% after PKA phosphorylation or in the presence of Ile5236Thr‐ahnak peptide. On native cardiomyocytes, intracellular application of this mutated ahnak peptide mimicked the PKA‐effects on I CaL increasing the amplitude by ≈60% and slowing its inactivation together with a leftward shift of its voltage dependency. Both mutated Ile5236Thr‐peptide and Ile5236Thr‐fragment (aa 5215‐5288) prevented specifically the further up‐regulation of I CaL by isoprenaline. Hence, we suggest the ahnak‐C1 domain serves as physiological brake on I CaL . Relief from this inhibition is proposed as common pathway used by sympathetic signaling and Ile5236Thr‐ahnak fragments to increase I CaL . This genetic ahnak variant might cause individual differences in I CaL regulation upon physiological challenges or therapeutic interventions.—Haase, H., Alvarez, J., Petzhold, D., Doller, A., Behlke, J., Erdmann, J., Hetzer, R., Regitz‐Zagrosek, V., Vassort, G., Morano, I. Ahnak is critical for cardiac Ca(v)1.2 calcium channel function and its β‐adrenergic regulation. FASEB J. 19, 1969–1977 (2005)