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Purinoceptors are involved in the induction of an osmolyte permeability in malaria‐infected and oxidized human erythrocytes
Author(s) -
Tanneur Valérie,
Duranton Christophe,
Brand Verena B.,
Sandu Ciprian D.,
Akkaya Canan,
Kasinathan Ravi S.,
Gachet Christian,
Sluyter Ronald,
Barden Julian A.,
Wiley James S.,
Lang Florian,
Huber Stephan M.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.04-3371fje
Subject(s) - osmolyte , purinergic receptor , plasmodium berghei , in vivo , autocrine signalling , microbiology and biotechnology , biology , chemistry , biochemistry , extracellular , pharmacology , immunology , receptor , malaria
In human erythrocytes, infection by the malaria parasite Plasmodium falciparum or oxidative stress induces a new organic osmolyte and anion permeability. To examine a role for autocrine purinoceptor signaling during this induction process, erythrocytic purinoceptor expression, and ATP release were determined. Furthermore, using pharmacological and genetic approaches the dependence on purinoceptor signaling of osmolyte permeability and Plasmodium development, both in vitro and in vivo, were assessed. Extracellular ATP did not induce an osmolyte permeability in non‐infected or non‐oxidized erythrocytes. ATP and other purinoceptor agonists increased the induction of osmolyte permeability during infection or oxidation as measured by isosmotic hemolysis and patch‐clamp recording. Purinoceptor antagonists and apyrase decreased the induced permeability. The observed pharmacology suggested the involvement of P2Y purinoceptors. Accordingly, human erythrocytes expressed P2Y 1 protein. Moreover, P2Y 1 deficient mouse erythrocytes exhibited a delayed appearance of the osmolyte permeability during P. berghei infection‐ or oxidation compared with wild‐type erythrocytes. Furthermore, the nonspecific purinoceptor antagonist suramin decreased in vitro growth and DNA/RNA amplification of P. falciparum in human erythrocytes and decreased in vivo growth of P. berghei . P. berghei developed slower in P2Y 1 ‐deficient mice in vivo compared with wild‐type animals. In conclusion, induction of the osmolyte permeability in Plasmodium ‐infected erythrocytes involves autocrine purinoceptor signaling.