z-logo
Premium
Cigarette smoke metal‐catalyzed protein oxidation leads to vascular endothelial cell contraction by depolymerization of microtubules
Author(s) -
Bernhard David,
Csordas Adam,
Henderson Blair,
Rossmann Andrea,
Kind Michaela,
Wick Georg
Publication year - 2005
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.04-3192com
Subject(s) - microtubule , microbiology and biotechnology , chemistry , endothelium , cytoskeleton , endothelial stem cell , intracellular , depolymerization , biophysics , biochemistry , cell , biology , in vitro , endocrinology , organic chemistry
Smoking is a significant risk factor for development of atherosclerosis. However, the pathophysiology of smoking‐mediated vessel wall damage is not understood. With tools ranging from analytical chemistry to cell biology, we show that cigarette smoke contains metals that catalyze the direct oxidation of cellular proteins by smoke oxidants. Oxidation of cellular proteins causes a loss of microtubule function, culminating in microtubule depolymerization and proteasome‐dependent degradation of α‐tubulin. As a consequence of the microtubule collapse, cytoskeletal structures as well as intermediate filaments break down, leading finally to a contraction of vascular endothelial cells. We observed a smoke extract‐induced, calpain‐dependent degradation of the intracellular form of platelet‐endothelial cell adhesion molecule 1/CD31, as well as a release of P‐selectin/CD62P, IL‐6, and IL‐8 from endothelial cells into the supernatant. Increased levels of soluble CD62P and IL‐6 are well known to be associated with smoking in humans. Increased permeability of the vascular endothelium is a crucial event in atherogenesis. This work highlights the compounds and mechanisms by which cigarette smoke induces leakiness of the vascular endothelium. DavidBernhard AdamCsordas BlairHenderson AndreaRossmann MichaelaKind GeorgWick Cigarette smoke metal‐catalyzed protein oxidation leads to vascular endothelial cell contraction by depolymerization of microtubules. FASEB J. 19, 1–12 (2005)

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here