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Novel role of microtubules in thrombin‐induced endothelial barrier dysfunction
Author(s) -
Birukova Anna A.,
Birukov Konstantin G.,
Smurova Ksenya,
Adyshev Djanybek,
Kaibuchi Kozo,
Alieva Irina,
Garcia Joe G. N.,
Verin Alexander D.
Publication year - 2004
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.04-2328com
Subject(s) - rhoa , thrombin , microbiology and biotechnology , heterotrimeric g protein , cytoskeleton , phosphorylation , rho associated protein kinase , actin cytoskeleton , chemistry , g protein , biology , signal transduction , cell , biochemistry , immunology , platelet
Disturbances in endothelial cell (EC) barrier regulation are critically dependent upon rearrangements of EC actin cytoskeleton. However, the role of microtubule (MT) network in the regulation of EC permeability is not well understood. We examined involvement of MT remodeling in thrombin‐induced EC permeability and explored MT regulation by heterotrimeric G12/13 proteins and by small GTPase Rho. Thrombin induced phosphorylation of MT regulatory protein tau at Ser 409 and Ser 262 and peripheral MT disassembly, which was linked to increased EC permeability. MT stabilization by taxol attenuated thrombin‐ induced permeability, actin remodeling, and paracellular gap formation and diminished thrombin‐induced activation of Rho and Rho‐kinase. Expression of activated Gα12/13 subunits involved in thrombin‐mediated signaling or their effector p115RhoGEF involved in Rho activation caused MT disassembly, whereas p115RhoGEF‐specific negative regulator RGS preserved MT from thrombin‐induced disassembly. Consistent with these results, expression of activated RhoA and Rho‐kinase induced MT disassembly. Conversely, thrombin‐induced disassembly of peripheral MT network was attenuated by expression of dominant negative RhoA and Rho‐kinase mutants or by pharmacological inhibition of Rho‐kinase. Collectively, our data demonstrate for the first time a critical involvement of MT disassembly in thrombin‐induced EC barrier dysfunction and indicate G‐protein‐dependent mechanisms of thrombin‐induced MT alteration.—Birukova, A. A., Birukov, K G., Smurova, K., Adyshev, D., Kaibuchi, K., Alieva, I., Garcia, J. G. N., Verin, A. D. Novel role of microtubules in thrombin‐induced endothelial barrier dysfunction. FASEB J. 18, 1879‐1890 (2004)