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The importance of virus‐associated host ICAM‐1 in human immunodeficiency virus type 1 dissemination depends on the cellular context
Author(s) -
Bounou Salim,
Giguère Jean-François,
Cantin Réjean,
Gilbert Caroline,
Imbeault Michael,
Martin Geneviève,
Tremblay Michel. J.
Publication year - 2004
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.04-1755fje
Subject(s) - biology , virus , context (archaeology) , virology , cell culture , microbiology and biotechnology , genetics , paleontology
The primary objective of this study was to define whether the nature of virion‐bound host cell membrane proteins influenced the process of human immunodeficiency virus 1 (HIV‐1) capture and transmission. We pulsed cells of monocytoid lineage (established and primary) and CD4‐ negative epithelial cells transiently expressing DC‐SIGN or LFA‐1 with isogenic HIV‐1 particles either devoid or bearing host‐derived ICAM‐1 or ICAM‐3 before incubation with an indicator cell line. To our surprise, the ICAM‐1/LFA‐1 association was a more efficient transmission factor than the combined gp120/DC‐SIGN and ICAM‐3/DC‐SIGN interactions. The involvement of the association between virus‐bound ICAM‐1 and its natural ligand LFA‐1 in virus binding and carriage was confirmed when using more physiological cellular targets, i.e., human lymphoid tissues cultured ex vivo. However, the contribution of virus‐anchored host ICAM‐1 to the process of retention and transmission of HIV‐1 could not be confirmed when using primary human cells of macrophage/dendritic lineage as transmitter cells and autologous CD4 + T lymphocytes as targets. Altogether these data underscore the complexity of factors participating in virus‐cell contact and efficient dissemination of HIV‐1 to target cells.