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Novel interaction partners of the TPR/MET tyrosine kinase
Author(s) -
Schaaf Christian P.,
Benzing Jörg,
Schmitt Thomas,
Erz Dorothee H. R.,
Tewes Magdalena,
Bartram Claus R.,
Janssen Johannes W. G.
Publication year - 2005
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.04-1558fje
Subject(s) - intracellular , microbiology and biotechnology , immunoprecipitation , tyrosine kinase , receptor tyrosine kinase , biology , two hybrid screening , sorting nexin , signal transduction , yeast , cell culture , biochemistry , genetics , endosome
A large variety of biological processes is mediated by stimulation of the receptor tyrosine kinase MET. Screening a mouse embryo cDNA library, we were able to identify several novel, putative intracellular TPR/MET‐substrates: SNAPIN, DCOHM, VAV‐1, Sorting nexin 2, Death associated protein kinase 3, SMC‐1, Centromeric protein C, and hTID‐1. Interactions as identified by yeast two‐hybrid analysis were validated in vitro and in vivo by mammalian two‐hybrid studies, a far‐western assay and coimmunoprecipitation. Participation in apoptosis‐regulating mechanisms through interaction with DAPK‐3 and cell cycle control via binding to nuclear proteins such as CENPC and SMC‐1 are possible new aspects of intracellular MET signaling.