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Platelet adhesion and signaling induced by the octapeptide primary binding sequence (KOGEOGPK) from type III collagen
Author(s) -
Maurice Pascal,
Legrand Chantal,
FauvelLafeve Francoise
Publication year - 2004
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.03-1151com
Subject(s) - tyrosine phosphorylation , platelet , phosphorylation , tyrosine , chemistry , platelet adhesiveness , adhesion , microbiology and biotechnology , integrin , collagen receptor , platelet membrane glycoprotein , gpvi , receptor , biochemistry , biology , immunology , platelet aggregation , organic chemistry
Platelet adhesion to vascular collagens is an essential step in the initiation of hemostasis and thrombosis. Several platelet receptors interact with type I and type III collagens, including GP Ia/IIa and GP VI. We recently described a new platelet receptor (TIIICBP) specific for a type III collagen‐related pri‐mary binding sequence, the KOGEOGPK octapeptide. Here, we characterize platelet adhesion to the immobi‐lized octapeptide and demonstrate that this adhesion 1 ) is Ca 2+ and Mg 2+ independent, suggesting a nonin‐volvement of GP Ia/IIa; 2 ) is not inhibited by an antibody against GP VI; and 3 ) triggers platelet protein tyrosine phosphorylation. Whereas TXA 2 has minimal effects, released ADP via only P2Y 12 potentiates plate‐let adhesion to the octapeptide. Octapeptide‐induced platelet adhesion triggers platelet signaling through tyrosine phosphorylation of the 68 kDa subunit of TIIICBP, Syk, PLCgamma2, and FAK. Tyrosine phos‐phorylation of the FcR gamma‐chain and LAT is also observed but to a lesser extent than with type III collagen, suggesting the requirement of GP VI for full tyrosine phosphorylation of FcR gamma‐chain and LAT. The present study provides evidence for a critical role for the type III collagen‐related KOGEOGPK octapeptide in mediating platelet adhesion and signal‐ing, and consequently in platelet‐collagen interactions.—Maurice, P., Legrand, C., Fauvel‐Lafeve, F. Platelet adhesion and signaling induced by the octapep‐tide primary binding sequence (KOGEOGPK) from type III collagen. FASEB J . 18, 1339–1347 (2004)