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Immunohistochemical detection of interleukin‐6 in human skeletal muscle fibers following exercise
Author(s) -
Penkowa Milena,
Keller Charlotte,
Keller Pernille,
Jauffred Sune,
Pedersen Bente Klarlund
Publication year - 2003
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.03-0311fje
Subject(s) - skeletal muscle , skeletal muscle fibers , immunohistochemistry , medicine , endocrinology , physical exercise , myocyte , chemistry
Interleukin‐6 (IL‐6) is produced by many different cell types. Human skeletal muscles produce and release high amounts of IL‐6 during exercise; however, the cell source of origin in the muscle is not known. Therefore, we studied the protein expression of IL‐6 by immunohistochemistry in human muscle tissue from biopsies obtained at time points 0, 3, 4.5, 6, 9, and 24 h in relation to 3 h of bicycle exercise performed by healthy young males ( n =12) and in resting controls ( n =6). The IL‐6 expression was clearly increased after exercise and remained high even by 24 h, relative to pre‐exercise or resting individuals. The IL‐6 immunostainings of skeletal muscle cells were homogeneous and without difference between muscle fiber types. The IL‐6 mRNA peaked immediately after the exercise, and, in accordance, the IL‐6 protein expression within muscle cells was most pronounced around 3 h post‐exercise. However, the finding that plasma IL‐6 concentration peaked in the end of exercise indicates a high turnover of muscle‐derived IL‐6. In conclusion, the finding of marked IL‐6 protein expression exclusively within skeletal muscle fibers following exercise demonstrates that skeletal muscle fibers of all types are the dominant cell source of exercise‐induced release of IL‐6 from working muscle.