Increases in weight of growth hormone‐deficient and immunodeficient (lit/scid) dwarf mice after grafting of hGH‐ secreting, primary human keratinocytes | Zendy

Bellini Maria Helena | Zendy; Peroni Cibele Nunes | Zendy; Bartolini Paolo | Zendy

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Increases in weight of growth hormone‐deficient and immunodeficient (lit/scid) dwarf mice after grafting of hGH‐ secreting, primary human keratinocytes

Author(s) -

Bellini Maria Helena,

Peroni Cibele Nunes,

Bartolini Paolo

Publication year - 2003

Publication title -

the faseb journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.709

H-Index - 277

eISSN - 1530-6860

pISSN - 0892-6638

DOI - 10.1096/fj.03-0018fje

Subject(s) - genetic enhancement , severe combined immunodeficiency , hormone , biology , body weight , skin grafting , gene , human growth hormone , medicine , growth hormone , endocrinology , microbiology and biotechnology , genetics , surgery

Primary human keratinocytes, stably transduced with the human growth hormone (hGH) gene (under control of the retroviral LTR promoter) and selected via geneticin secreted as much as 7 μg hGH/10 6 cells/day. Their grafting onto immunodeficient dwarf mice (lit/scid) led to hGH levels in the circulation that did not go below 0.2‐0.3 ng/ml during a 12 day period (peak value, 1.5 ng/ml at 4 h). This phenomenon was associated with a body weight increase of the grafted animals (0.060 g/animal/day) significantly higher ( P <0.01) than that of controls (0.023 g/animal/day). This is the first report describing successful utilization of immunodeficient dwarf mice (lit/scid) in keratinocyte‐based hGH gene therapy.

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