Premium
Monocyte recruitment to endothelial cells in response to oscillatory shear stress
Author(s) -
Hsiai Tzung K.,
Cho Sung K.,
Wong Pak K.,
Ing Mike,
Salazar Adler,
Sevanian Alex,
Navab Mohamad,
Demer Linda L.,
Ho Chih-Ming
Publication year - 2003
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.02-1064com
Subject(s) - monocyte , shear stress , shear (geology) , biophysics , cell adhesion molecule , chemistry , microbiology and biotechnology , mechanics , materials science , physics , biology , immunology , composite material
Leukocyte recruitment to endothelial cells is a critical event in inflammatory responses. The spatial, temporal gradients of shear stress, topology, and outcome of cellular interactions that underlie these responses have so far been inferred from static imaging of tissue sections or studies of statically cultured cells. In this report, we developed micro‐electromechanical systems (MEMS) sensors, comparable to a single endothelial cell (EC) in size, to link real‐time shear stress with monocyte/EC binding kinetics in a complex flow environment, simulating the moving and unsteady separation point at the arterial bifurcation with high spatial and temporal resolution. In response to oscillatory shear stress (τ) at ± 2.6 dyn/cm 2 at a time‐averaged shear stress (τ ave ) = 0 and 0.5 Hz, individual monocytes displayed unique to‐and‐fro trajectories undergoing rolling, binding, and dissociation with other monocyte, followed by solid adhesion on EC. Our study quantified individual monocyte/EC binding kinetics in terms of displacement and velocity profiles. Oscillatory flow induces up‐regulation of adhesion molecules and cytokines to mediate monocyte/EC interactions over a dynamic range of shear stress ± 2.6 dyn/cm 2 ( P =0.50. n =10).—Hsiai, T. K., Cho, S. K., Wong, P. K., Ing, M., Salazar, A., Sevanian, A., Navab, M., Demer, L. L., Ho, C.‐M. Monocyte recruitment to endothelial cells in response to oscillatory shear stress. FASEB J. 17, 1648–1657 (2003)