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Compartmentalization of bicarbonate‐sensitive adenylyl cyclase in distinct signaling microdomains
Author(s) -
Zippin Jonathan H.,
Chen Yanqiu,
Nahirney Patrick,
Kamenetsky Margarita,
Wuttke Mark S.,
Fischman Donald A.,
Levin Lonny R.,
Buck Jochen
Publication year - 2003
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fj.02-0598fje
Subject(s) - adenylyl cyclase , compartmentalization (fire protection) , camp dependent pathway , microbiology and biotechnology , adcy10 , intracellular , gs alpha subunit , second messenger system , adcy9 , biology , chemistry , signal transduction , biochemistry , enzyme
Intracellular targets of the ubiquitous second messenger cAMP are located at great distances from the most widely studied source of cAMP, the G protein responsive transmembrane adenylyl cyclases. We previously identified an alternative source of cAMP in mammalian cells lacking transmembrane spanning domains, the “soluble” adenylyl cyclase (sAC). We now demonstrate that sAC is distributed in specific subcellular compartments: mitochondria, centrioles, mitotic spindles, mid‐bodies, and nuclei, all of which contain cAMP targets. Distribution at these intracellular sites proves that adenylyl cyclases are in close proximity to all cAMP effectors, suggesting a model in which local concentrations of cAMP are regulated by individual adenylyl cyclases targeted to specific microdomains throughout the cell.